| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gregorace, Sophia R (12)
|
MRLIAC |
36.12 |
36.94 |
0.82 |
| 2 |
Kern, Margo M (12)
|
MRLIAC |
40.16 |
38.93 |
-1.23 |
| 3 |
Nierhoff, Luisa (11)
|
MRBGNW |
38.26 |
40.95 |
2.69 |
| 4 |
Chu, Sophia W (12)
|
MRLIAC |
40.78 |
41.35 |
0.57 |
| 5 |
Gary, Isabella S (12)
|
CTWYW |
41.26 |
41.38 |
0.12 |
| 6 |
Lim, Jessica G (12)
|
MRLGAC |
41.09 |
41.93 |
0.84 |
| 7 |
Arciniegas, Francesca (11)
|
MRCOND |
41.40 |
42.14 |
0.74 |
| 8 |
Vandermel, Katherine E (12)
|
MRCOND |
41.80 |
43.01 |
1.21 |
| 9 |
Cornish, Kristin A (11)
|
MRBGNW |
40.24 |
43.81 |
3.57 |
| 10 |
Gamble, Kathryn L (12)
|
MRTS |
41.68 |
44.18 |
2.50 |
| 11 |
Paez, Isabella S (11)
|
MRBGNW |
42.08 |
44.99 |
2.91 |